Breastfeeding Research Today is a free monthly online journal that collates and summarizes the latest research about Breastfeeding, including details on breast feeding benefits, problems, alcohol, diet. | ||||||||
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Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools.Nevoral J, Rada V, Vlková E, Bláhová K, Bronský J, Bubáková D, Killer J Department of Pediatrics, 2nd Medical School of the Charles University in Prague and University Hospital Motol, 150 06, Prague, Czech Republic. jiri.nevoral@seznam.cz Intestinal microbiota in exclusively breast-fed infants with blood-streaked stools and in healthy exclusively breast-fed babies was compared. Total anaerobes, bifidobacteria, lactobacilli, coliform bacteria, enterococci and clostridia were quantified by cultivation methods in feces of 17 full-term exclusively breastfed patients (aged 16.3 +/- 7.4 weeks) with blood-streaked stools and in the control group of 22 healthy fullterm exclusively breast-fed infants (13.7 +/- 6.4 weeks). Specific fluorescence in situ hybridization kits for Bifidobacterium spp. were used for the quantitative detection of bifidobacteria in samples. Control samples had significantly (p < 0.05) higher counts of total anaerobes. Bifidobacteria were not detected in patients' samples in 65 % and in controls in 36 % (p < 0.01). Bifidobacteria counts were also significantly higher in the control group (p < 0.01). Furthermore, clostridia strains were detected only in feces from bifidobacteria-negative infants reaching counts >8 log CFU/g. Lactobacilli were not detected in 65 % patients and in 45 % control samples. However, this difference was not significant as well as the difference in lactobacilli counts. Eosinophilia was observed in 35 % of patients, low IgA concentration in 71 % and also low IgG concentration in 71 %. pANCA positivity was found in 53 % of patients. In conclusion a significant low proportion of bifidobacterial microbiota in patients with blood-streaked stools was shown in comparison with controls. Published 6 May 2009 in Folia Microbiol (Praha), 54(2): 167-71.
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